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Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

SKU:
MODL00962
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64669
Sensitivity:
0.127ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
DHQU,DIA4,DTD,NMOR1,NMORI,QR1,Azoreductase,DT-diaphorase,Menadione reductase,Phylloquinone reductase,Quinone reductase 1,NAD(P)H:quinone oxidoreductase 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
Frequently bought together:

Description

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Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

The Mouse NADH Dehydrogenase Quinone 1 (NQO1) ELISA Kit is an essential tool for researchers studying the role of NQO1 in various biological processes. This kit is specifically designed for the accurate and sensitive detection of NQO1 levels in mouse serum, plasma, and tissue homogenates.NQO1 is a key enzyme involved in the detoxification of quinones and protecting cells from oxidative stress. Dysregulation of NQO1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.

With high sensitivity and specificity, this ELISA kit provides reliable and reproducible results, making it an ideal choice for a wide range of research applications. By accurately measuring NQO1 levels, researchers can gain valuable insights into the role of this enzyme in disease pathogenesis and potentially develop targeted therapies.

Product Name:Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit
Product Code:MODL00962
Size:96 Assays
Target:NQO1
Synonyms:DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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