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Mouse N-Acetyltransferase 1 (NAT1) ELISA Kit

SKU:
MODL00921
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62448
Sensitivity:
0.066ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AAC1,Arylamine N-Acetyltransferase,Arylamide acetylase 1,Monomorphic arylamine N-acetyltransferase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse N-Acetyltransferase 1 (NAT1) ELISA Kit

The Mouse N-Acetyltransferase 1 (NAT1) ELISA Kit is specifically designed for the precise measurement of NAT1 levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring dependable and consistent results, which makes it perfect for a variety of research purposes.N-Acetyltransferase 1 (NAT1) is a key enzyme involved in the metabolism of xenobiotics and drugs in mice, playing a critical role in drug efficacy and toxicity.

Dysregulation of NAT1 has been linked to various diseases and conditions, making it a valuable biomarker for studying these conditions and developing potential treatments.Don't miss out on the opportunity to accurately measure NAT1 levels in mouse samples with the Mouse N-Acetyltransferase 1 (NAT1) ELISA Kit. Order yours today and take your research to the next level.

Product Name:Mouse N-Acetyltransferase 1 (NAT1) ELISA Kit
Product Code:MODL00921
Size:96 Assays
Target:NAT1
Synonyms:AAC1, Arylamine N-Acetyltransferase, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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