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Mouse Myostatin (MSTN) ELISA Kit

SKU:
MODL00892
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O08689
Sensitivity:
35pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
GDF8,Growth Differentiation Factor 8
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Myostatin (MSTN) ELISA Kit

The Mouse Myostatin (MSTN) ELISA Kit is specifically designed for the sensitive and accurate measurement of myostatin levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high specificity and precision, ensuring consistent and reliable results that are essential for a variety of research applications.Myostatin is a key regulator of muscle growth and development, inhibiting muscle cell proliferation and differentiation.

Dysregulation of myostatin has been implicated in conditions such as muscle wasting disorders and obesity, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. This ELISA kit provides researchers with a powerful tool to study the role of myostatin in muscle physiology and pathophysiology, ultimately advancing our understanding of muscle-related disorders.

Product Name:Mouse Myostatin (MSTN) ELISA Kit
Product Code:MODL00892
Size:96 Assays
Target:MSTN
Synonyms:GDF8, Growth Differentiation Factor 8
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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