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Mouse Myosin Heavy Chain 4, Skeletal Muscle (MYH4) ELISA Kit

SKU:
MODL00912
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5SX39
Sensitivity:
7.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
MYH2B,Myosin heavy chain 2b,Myosin heavy chain, skeletal muscle, fetal
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Myosin Heavy Chain 4, Skeletal Muscle (MYH4) ELISA Kit

The Mouse Myosin Heavy Chain 4 (Skeletal Muscle) (MYH4) ELISA Kit from Assay Genie is a powerful tool for researchers studying skeletal muscle development and function in mice. This kit allows for the precise quantification of MYH4 levels in mouse serum, plasma, and tissue lysates with high sensitivity and accuracy.MYH4 is a key protein involved in muscle contraction and movement, making it essential for understanding skeletal muscle physiology and pathophysiology.

By measuring MYH4 levels, researchers can gain valuable insights into muscle growth, regeneration, and diseases such as muscular dystrophy.With its reliable performance and user-friendly protocol, the Mouse MYH4 ELISA Kit is suitable for a wide range of research applications in the fields of muscle biology, exercise physiology, and drug development. Stay ahead in your research with the Assay Genie Mouse MYH4 ELISA Kit.

Product Name:Mouse Myosin Heavy Chain 4, Skeletal Muscle (MYH4) ELISA Kit
Product Code:MODL00912
Size:96 Assays
Target:MYH4
Synonyms:MYH2B, Myosin heavy chain 2b, Myosin heavy chain, skeletal muscle, fetal
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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