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Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) ELISA Kit

SKU:
MODL00911
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
G3UW82
Sensitivity:
6.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
MYH2A,MYHSA2,MYHas8,MyHC-2A,MyHC-IIa
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) ELISA Kit

The Mouse Myosin Heavy Chain 2 Skeletal Muscle (MYH2) ELISA Kit is specifically designed for the accurate measurement of MYH2 levels in adult mouse skeletal muscle tissues. This kit offers high sensitivity and specificity, ensuring precise and consistent results for use in various research applications.MYH2 is an important muscle protein that plays a crucial role in muscle contraction and movement. It is essential for maintaining muscle structure and function, making it a valuable marker for studying skeletal muscle development, function, and diseases.

With its reliable performance and ease of use, the Mouse MYH2 ELISA Kit is an indispensable tool for researchers studying muscle physiology, exercise science, and musculoskeletal disorders. Get accurate and reliable results with this advanced ELISA kit from Assay Genie.

Product Name:Mouse Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2) ELISA Kit
Product Code:MODL00911
Size:96 Assays
Target:MYH2
Synonyms:MYH2A, MYHSA2, MYHas8, MyHC-2A, MyHC-IIa
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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