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Mouse Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

SKU:
MODL00847
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97287
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
EAT,MCL1L,MCL1S,BCL2L3,TM,Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1,Bcl-2-like protein 3,Bcl-2-related protein EAT/mcl1,mcl1/EAT
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

The Mouse Myeloid Cell Leukemia Sequence 1 (Mcl-1) ELISA Kit is a comprehensive tool for measuring levels of Mcl-1 in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and dependable results that are perfect for a variety of research endeavors.Mcl-1 is a vital protein involved in regulating cell survival, making it a key player in various cellular processes such as apoptosis and proliferation. Dysregulation of Mcl-1 has been implicated in diseases like leukemia and other cancers, underscoring its importance as a biomarker for disease progression and potential therapeutic interventions.

With the Mouse Myeloid Cell Leukemia Sequence 1 (Mcl-1) ELISA Kit, researchers can accurately quantify Mcl-1 levels in various biological samples, providing valuable insights into the role of this protein in cellular physiology and disease pathology. Order this kit today and unlock new possibilities in your research.

Product Name:Mouse Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit
Product Code:MODL00847
Size:96 Assays
Target:MCL1
Synonyms:EAT, MCL1L, MCL1S, BCL2L3, TM, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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