The Mouse MUC5AC (Mucin 5 Subtype AC) ELISA Kit from Assay Genie is specifically designed for the accurate quantification of MUC5AC levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MUC5AC is a mucin protein that plays a key role in mucin secretion, mucociliary clearance, and protection of the respiratory tract.
Abnormal expression of MUC5AC is associated with various respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis. This ELISA kit provides researchers with a valuable tool for studying the role of MUC5AC in respiratory diseases and exploring potential therapeutic strategies.
Product Name:
Mouse MUC5AC (Mucin-5 subtype AC) ELISA Kit
SKU:
MOES01275
Target:
Mouse MUC5AC (Mucin-5 subtype AC)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC5AC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MUC5AC. You can calculate the concentration of Mouse MUC5AC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
92-104
86-102
Average (%)
95
98
93
1:4
Range (%)
94-106
85-99
83-97
Average (%)
99
92
89
1:8
Range (%)
91-107
81-93
88-99
Average (%)
98
86
93
1:16
Range (%)
87-102
81-91
85-99
Average (%)
94
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
95-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.56
3.44
0.54
1.53
3.65
Standard deviation
0.03
0.06
0.11
0.03
0.09
0.17
C V (%)
5.88
3.85
3.2
5.56
5.88
4.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MUC5AC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MUC5AC in samples. No significant cross-reactivity or interference between Mouse MUC5AC and analogues was observed.
