The Mouse MUC1 (Mucin 1) ELISA Kit is specifically designed for the precise measurement of MUC1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.MUC1 is a key glycoprotein that plays a vital role in cell adhesion and signaling, particularly in epithelial tissues. Abnormal expression of MUC1 has been associated with various diseases such as cancer, inflammatory conditions, and autoimmune disorders, making it a valuable biomarker for studying these pathologies and potential therapeutic interventions.
With its reliable performance and easy-to-use format, the Mouse MUC1 ELISA Kit is an essential tool for researchers investigating the role of MUC1 in disease progression and exploring potential treatment options. Elevate your research with this advanced ELISA kit from Assay Genie.
Product Name:
Mouse MUC1 (Mucin 1) ELISA Kit
SKU:
MOES01746
Target:
Mouse MUC1 (Mucin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MUC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MUC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MUC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MUC1. You can calculate the concentration of Mouse MUC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
89-103
90-103
Average (%)
93
94
95
1:4
Range (%)
93-109
80-94
86-102
Average (%)
99
86
93
1:8
Range (%)
88-100
85-99
83-96
Average (%)
93
92
89
1:16
Range (%)
87-102
81-92
87-98
Average (%)
93
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.89
4.96
0.53
0.87
4.99
Standard deviation
0.03
0.04
0.22
0.03
0.05
0.25
C V (%)
6.12
4.49
4.44
5.66
5.75
5.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MUC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MUC1 in samples. No significant cross-reactivity or interference between Mouse MUC1 and analogues was observed.
