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Mouse αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit (AEES00198)

SKU:
AEES00198
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Mouse
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

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Product Name:Mouse αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit
SKU:AEES00198
Target:Mouse αMSH (Alpha-Melanocyte Stimulating Hormone)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.5h
Sensitivity:9.38 pg/mL
Detection range:15.63-1000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with αMSH. During the reaction, αMSH in the sample or standard competes with a fixed amount of αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to αMSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of αMSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)83-9698-11084-95
Average (%)9010292
1:4Range (%)87-9789-9885-96
Average (%)919390
1:8Range (%)97-10593-10588-95
Average (%)1029891
1:16Range (%)87-10195-10595-104
Average (%)93100101
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)92-10599
EDTA plasma (n=5)95-10198
Cell culture media (n=5)89-10297
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)49.64113.98406.6440.1119.5414.0
Standard deviation2.695.322.082.255.4717.06
C V (%)5.424.655.435.614.584.12
Sample type &Sample volume:serum, plasma and other biological fluids; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of αMSH concentrations in serum, plasma and other biological fluids.
Specificity:This kit recognizes αMSH in samples. No significant cross-reactivity or interference between αMSH and analogues was observed.

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