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Mouse Monocyte Chemotactic Protein 5 (MCP5) ELISA Kit

SKU:
MODL00851
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q62401
Sensitivity:
5.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL12,SCYA12,Chemokine C-C-Motif Ligand 12,MCP-1-related chemokine,Monocyte chemoattractant protein 5,Small-inducible cytokine A12
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Mouse Monocyte Chemotactic Protein 5 (MCP5) ELISA Kit

The Mouse Monocyte Chemotactic Protein-5 (MCP5) ELISA Kit is designed for the precise measurement of MCP5 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MCP5 is a vital chemokine involved in recruiting monocytes to sites of inflammation and injury, playing a crucial role in the immune response. Studies have shown that dysregulation of MCP5 levels can contribute to various inflammatory diseases, making it an important biomarker for researching and understanding these conditions.

Utilizing this MCP5 ELISA Kit can provide valuable insights into the role of MCP5 in immune responses and inflammation, aiding in the development of potential therapeutic strategies for inflammatory diseases. Get accurate and reliable data with this high-quality ELISA kit from Assay Genie.

Product Name:Mouse Monocyte Chemotactic Protein 5 (MCP5) ELISA Kit
Product Code:MODL00851
Size:96 Assays
Target:MCP5
Synonyms:CCL12, SCYA12, Chemokine C-C-Motif Ligand 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Small-inducible cytokine A12
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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