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Mouse MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

SKU:
AEES00245
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Mouse
Sensitivity:
0.94 ng/mL
Range:
1.56-100 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Mouse MMP-9 (Matrix Metalloproteinase 9) ELISA Kit

The Mouse MMP-9 (Matrix Metalloproteinase 9) ELISA Kit is a cutting-edge assay meticulously crafted for the precise quantitative assessment of MMP-9 levels in diverse biological samples. MMP-9, a critical member of the matrix metalloproteinase family, plays a pivotal role in cellular activities including extracellular matrix remodeling, cell migration, tissue repair, and immune response modulation. Its regulatory functions extend to processes such as angiogenesis, wound healing, and inflammation, making it a key player in various physiological and pathological conditions. Accurate measurement of MMP-9 concentrations is crucial for elucidating its involvement in disease mechanisms, particularly in conditions like cancer metastasis, tissue fibrosis, and cardiovascular diseases where MMP-9 dysregulation is implicated. This ELISA kit empowers researchers to explore the multifaceted functions of MMP-9 in different biological contexts, providing valuable insights into its impact on cellular behavior, tissue homeostasis, and disease progression.

Equipped with exceptional sensitivity and specificity, the Mouse MMP-9 ELISA Kit by Assay Genie ensures robust and reproducible results, enabling researchers to unravel the complex roles of MMP-9 in health and disease. Manufactured under stringent quality control standards, this kit offers reliable performance and straightforward protocols, making it an indispensable tool for research endeavors seeking to delve into the functional significance of MMP-9 in diverse biological processes. Opt for Assay Genie's MMP-9 ELISA Kit to advance your investigations and gain deeper insights into the intricate functions of Matrix Metalloproteinase 9.

Product Name:Mouse MMP-9 (Matrix Metalloproteinase 9) ELISA Kit
Product Code:AEES00245
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Mouse
Detection Range:1.56-100 ng/mL
Sensitivity:0.94 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Mouse MMP-9 in samples. No significant cross-reactivity or interference between Mouse MMP-9 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Mouse MMP-9 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-9. You can calculate the concentration of Mouse MMP-9 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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