The Mouse MIF (Macrophage Migration Inhibitory Factor) CLIA Kit is a highly sensitive assay designed for the accurate measurement of MIF levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Macrophage Migration Inhibitory Factor is a key cytokine involved in regulating immune response and inflammation.
It plays a crucial role in various physiological and pathological processes, such as autoimmune diseases, infections, and cancer. The Mouse MIF CLIA Kit allows researchers to study the role of MIF in these conditions and explore potential therapeutic interventions.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MIF. You can calculate the concentration of Mouse MIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
91-104
91-102
Average (%)
94
98
97
1:4
Range (%)
102-119
93-105
86-101
Average (%)
110
99
93
1:8
Range (%)
91-104
100-116
86-98
Average (%)
99
109
93
1:16
Range (%)
102-119
99-111
83-96
Average (%)
109
106
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.18
50.8
183.34
22.79
47.95
176.46
Standard deviation
3.19
4.64
12.34
1.93
4.33
20.2
C V (%)
12.67
9.13
6.73
8.47
9.03
11.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MIF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIF in samples. No significant cross-reactivity or interference between Mouse MIF and analogues was observed.
