The Mouse MIγ/CXCL9 (Monocyte Interferon Gamma Inducing Factor) ELISA Kit is a sophisticated tool engineered for precise and quantitative measurement of MIγ/CXCL9 in various biological samples. MIγ/CXCL9, also known as Monocyte Interferon Gamma Inducing Factor, is a significant chemokine involved in immune responses and the recruitment of immune cells, particularly monocytes and T cells, to sites of inflammation. Accurate quantification of MIγ/CXCL9 levels is crucial for understanding its crucial role in mediating immune cell behaviors and regulating immune responses, particularly in the context of inflammatory processes and immune cell recruitment.
This ELISA kit provides researchers with a reliable method to analyze the expression levels of MIγ/CXCL9, enabling a deeper exploration of its involvement in immune-related pathways and disease states. Our Mouse MIγ/CXCL9 ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for your research needs. Manufactured with stringent quality control measures, this kit offers robust and reliable performance, making it an excellent choice for studies focusing on immune cell recruitment, inflammation, and immune response modulation.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MIγ/CXCL9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MIγ/CXCL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MIγ/CXCL9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MIγ/CXCL9. You can calculate the concentration of Mouse MIγ/CXCL9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
94-107
90-100
Average (%)
103
99
95
1:4
Range (%)
94-106
93-110
85-101
Average (%)
100
100
92
1:8
Range (%)
97-113
89-104
87-100
Average (%)
103
95
93
1:16
Range (%)
92-107
89-106
87-99
Average (%)
99
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.48
122.36
417.45
49.33
124.41
404.58
Standard deviation
3.6
5.64
15.95
3.31
7.4
19.46
C V (%)
6.61
4.61
3.82
6.71
5.95
4.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse MIγ/CXCL9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MIγ/CXCL9 in samples. No significant cross-reactivity or interference between Mouse MIγ/CXCL9 and analogues was observed.
