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Mouse Methyl CpG Binding Protein 2 (MECP2) ELISA Kit (MODL00855)

SKU:
MODL00855
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z2D6
Sensitivity:
0.102ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
RTS,AUTSX3,MRX16,MRX79,PPMX,RTT,Rett Syndrome,Mental Retardation,X-linked 16
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Methyl CpG Binding Protein 2 (MECP2) ELISA Kit

The Mouse Methyl-CpG Binding Protein 2 (MeCP2) ELISA Kit is specifically designed for the precise measurement of MeCP2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MeCP2 is a key protein involved in regulating gene expression by binding to methylated CpG sites on DNA. Dysregulation of MeCP2 has been implicated in neurodevelopmental disorders such as Rett Syndrome, making it a critical target for studying these conditions and exploring potential therapeutic interventions.

By using the Mouse MeCP2 ELISA Kit, researchers can gain valuable insights into the role of MeCP2 in neurodevelopment and disease progression, facilitating advancements in the field of neuroscience and potential treatments for neurodevelopmental disorders.

Product Name:Mouse Methyl CpG Binding Protein 2 (MECP2) ELISA Kit
Product Code:MODL00855
Size:96 Assays
Target:MECP2
Synonyms:RTS, AUTSX3, MRX16, MRX79, PPMX, RTT, Rett Syndrome, Mental Retardation,X-linked 16
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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