The Mouse MDC (CCL22) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of Macrophage-Derived Chemokine levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers studying immune responses, inflammation, and allergic reactions in mouse models.MDC (CCL22) is a chemokine that plays a critical role in recruiting immune cells to sites of inflammation and regulating the immune response.
Elevated levels of MDC have been associated with various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for understanding disease pathogenesis and developing targeted therapies. Overall, the Mouse MDC (CCL22) CLIA Kit offers researchers a dependable and efficient solution for studying the role of Macrophage-Derived Chemokine in mouse models, ultimately advancing our understanding of immune system function and disease processes.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MDC/CCL22. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MDC/CCL22 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MDC/CCL22, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MDC/CCL22. You can calculate the concentration of Mouse MDC/CCL22 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
101-118
89-103
Average (%)
93
110
95
1:4
Range (%)
100-117
100-116
95-108
Average (%)
108
108
101
1:8
Range (%)
102-117
92-107
93-110
Average (%)
109
100
101
1:16
Range (%)
103-116
99-111
87-99
Average (%)
109
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
96-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.02
61.56
202.14
24.92
55.44
193.92
Standard deviation
3.04
4.56
16.15
2.41
5.48
12.16
C V (%)
11.68
7.41
7.99
9.67
9.88
6.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MDC/CCL22 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MDC/CCL22 in samples. No significant cross-reactivity or interference between Mouse MDC/CCL22 and analogues was observed.
