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Mouse Matrix Metalloproteinase 7 (MMP7) ELISA Kit

SKU:
MODL00880
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q10738
Sensitivity:
26.5pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
MPSL1,MAT,PUMP-1,Matrilysin Uterine,Matrilysin,Matrin,Pump-1 Protease,Uterine Metalloproteinase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Matrix Metalloproteinase 7 (MMP7) ELISA Kit

The Mouse Matrix Metalloproteinase-7 (MMP7) ELISA Kit is specifically designed for the precise measurement of MMP7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are suitable for a variety of research purposes.MMP7 is an important enzyme that plays a critical role in tissue remodeling and extracellular matrix degradation. Dysregulation of MMP7 has been linked to various diseases, including cancer, inflammatory disorders, and fibrotic conditions.

Therefore, this ELISA kit serves as a valuable tool for studying the role of MMP7 in disease pathogenesis and potential therapeutic interventions.Overall, the Mouse Matrix Metalloproteinase-7 (MMP7) ELISA Kit provides researchers with a reliable and efficient method for quantifying MMP7 levels in biological samples, ultimately contributing to advancements in the understanding and treatment of MMP7-associated diseases.

Product Name:Mouse Matrix Metalloproteinase 7 (MMP7) ELISA Kit
Product Code:MODL00880
Size:96 Assays
Target:MMP7
Synonyms:MPSL1, MAT, PUMP-1, Matrilysin Uterine, Matrilysin, Matrin, Pump-1 Protease, Uterine Metalloproteinase
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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