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Mouse Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU:
MODL00879
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28862
Sensitivity:
0.148ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SL1,STMY,STMY1,STR1,Progelatinase,Stromelysin 1,Transin-1
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

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Mouse Matrix Metalloproteinase 3 (MMP3) ELISA Kit

The Mouse Matrix Metalloproteinase-3 (MMP3) ELISA Kit is specifically designed for the accurate detection of MMP3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results, suitable for various research applications.MMP3 is an important enzyme involved in the breakdown of extracellular matrix proteins, playing a key role in tissue remodeling and repair processes. Dysregulation of MMP3 has been implicated in various diseases such as arthritis, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.

With this MMP3 ELISA Kit, researchers can easily and efficiently quantitate MMP3 levels in mouse samples, leading to a deeper understanding of its role in disease pathogenesis and progression. Stay ahead in your research with this reliable and accurate kit from Assay Genie.

Product Name:Mouse Matrix Metalloproteinase 3 (MMP3) ELISA Kit
Product Code:MODL00879
Size:96 Assays
Target:MMP3
Synonyms:SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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