The Mouse Matrix Metalloproteinase-2 (MMP2) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of MMP2 levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it a valuable tool for research studies involving MMP2 and its role in various physiological processes.MMP2 is a key enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair. Dysregulation of MMP2 activity has been implicated in various pathologies, including cancer, cardiovascular diseases, and inflammatory conditions.
The ability to accurately measure MMP2 levels in biological samples can provide important insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and specificity, the Mouse MMP2 ELISA Kit offers researchers a convenient and efficient method for studying the role of MMP2 in health and disease. Whether investigating basic research questions or exploring potential treatment options, this kit is an essential tool for advancing scientific understanding in the field of matrix metalloproteinases.
Product Name:
Mouse Matrix Metalloproteinase 2 (MMP2) ELISA Kit
Product Code:
MODL00878
Size:
96 Assays
Target:
MMP2
Synonyms:
CLG4, CLG4A, MMP-II, MONA, TBE-1, PEX, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
