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Mouse Matrix Metalloproteinase 15 (MMP15) ELISA Kit

SKU:
MODL00876
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O54732
Sensitivity:
28.4pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
SMCP-2,MTMMP2,MT2-MMP,Membrane-Inserted,Membrane-type matrix metalloproteinase 2,Membrane-type-2 matrix metalloproteinase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Matrix Metalloproteinase 15 (MMP15) ELISA Kit

The Mouse Matrix Metalloproteinase 15 (MMP15) ELISA Kit is designed for the accurate detection of MMP15 levels in mouse serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.MMP15 is a key protein involved in the breakdown of extracellular matrix in tissues, playing a crucial role in processes such as tissue remodeling, wound healing, and tumor metastasis.

Its dysregulation has been linked to various diseases, including cancer, arthritis, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With this MMP15 ELISA Kit, researchers can easily quantify MMP15 levels in their samples, providing valuable insights into disease mechanisms and potential treatment strategies. Order your kit today and take your research to the next level.

Product Name:Mouse Matrix Metalloproteinase 15 (MMP15) ELISA Kit
Product Code:MODL00876
Size:96 Assays
Target:MMP15
Synonyms:SMCP-2, MTMMP2, MT2-MMP, Membrane-Inserted, Membrane-type matrix metalloproteinase 2, Membrane-type-2 matrix metalloproteinase
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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