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Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit

SKU:
MODL00875
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P53690
Sensitivity:
32.81pg/mL
Range:
93.75-6000pg/mL
ELISA Type:
Sandwich
Synonyms:
MMP-X1,MT1-MMP,MTMMP1,Membrane Inserted,Membrane-type matrix metalloproteinase 1,Membrane-type-1 matrix metalloproteinase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit

The Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit is a powerful tool for researchers looking to quantify MMP14 levels in mouse biological samples. This kit offers exceptional sensitivity and specificity, guaranteeing precise and dependable results for a variety of research purposes.MMP14 is a key enzyme involved in extracellular matrix remodeling and cell migration, playing a critical role in cancer progression, tissue repair, and inflammation.

By accurately measuring MMP14 levels, researchers can gain valuable insights into the mechanisms underlying these biological processes and potentially identify new therapeutic targets.Whether studying cancer metastasis, tissue development, or inflammatory responses, the Mouse MMP14 ELISA Kit provides researchers with a reliable and user-friendly solution for exploring the role of MMP14 in various physiological and pathological conditions.

Product Name:Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit
Product Code:MODL00875
Size:96 Assays
Target:MMP14
Synonyms:MMP-X1, MT1-MMP, MTMMP1, Membrane Inserted, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase
Detection Method:Sandwich
Range:93.75-6,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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