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Mouse Matrix Metalloproteinase 11 (MMP11) ELISA Kit

SKU:
MODL00872
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02853
Sensitivity:
0.08ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SL3,ST3,STMY3,Stromelysin 3
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Matrix Metalloproteinase 11 (MMP11) ELISA Kit

The Mouse Matrix Metalloproteinase-11 (MMP-11) ELISA Kit is a reliable tool for detecting MMP-11 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for various research applications.MMP-11 is a key enzyme involved in extracellular matrix remodeling, tissue repair, and tumor progression. Its dysregulation has been linked to various pathological conditions, including cancer, inflammation, and tissue fibrosis.

Studying MMP-11 levels can shed light on disease mechanisms and aid in the development of targeted therapies.Overall, the Mouse MMP-11 ELISA Kit is a valuable resource for researchers investigating the role of MMP-11 in health and disease, offering a comprehensive and easy-to-use solution for measuring its expression levels in biological samples.

Product Name:Mouse Matrix Metalloproteinase 11 (MMP11) ELISA Kit
Product Code:MODL00872
Size:96 Assays
Target:MMP11
Synonyms:SL3, ST3, STMY3, Stromelysin 3
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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