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Mouse Mannose Binding Lectin (MBL) ELISA Kit

SKU:
MODL00844
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P39039
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MBL2,COLEC1,HSMBPC,MBP1,MBP,Collectin-1,Mannose-binding protein C,Mannan Binding Protein,Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Mannose Binding Lectin (MBL) ELISA Kit

The Mouse Mannose Binding Lectin (MBL) ELISA Kit is specifically designed for the precise measurement of MBL levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for various research applications.MBL is a key protein involved in the innate immune response, playing a critical role in recognizing and binding to pathogens for immune defense. Studies have shown that MBL deficiencies are associated with increased susceptibility to infections and autoimmune diseases.

Therefore, measuring MBL levels in mouse models can provide valuable insights into immune responses and potential therapeutic approaches.Don't miss out on the chance to study the important role of MBL in immune regulation and disease pathogenesis with the Mouse Mannose Binding Lectin (MBL) ELISA Kit. Order yours today and advance your research in the field of immunology and infectious diseases.

Product Name:Mouse Mannose Binding Lectin (MBL) ELISA Kit
Product Code:MODL00844
Size:96 Assays
Target:MBL
Synonyms:MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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