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Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU:
MODL00867
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14097
Sensitivity:
4.3pg/mL
Range:
12.5-800pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL4,ACT2,G-26,LAG1,MIP1-B,SCYA4,Chemokine C-C-Motif Ligand 4,Small Inducible Cytokine A4,Homologous To Mouse Mip-1b
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

The Mouse Macrophage Inflammatory Protein 1 Beta (MIP1B) ELISA Kit is specifically designed for the accurate and sensitive detection of MIP1B levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring robust and reproducible results for a variety of research applications.MIP1B is a key cytokine involved in the inflammatory response, playing a critical role in recruiting immune cells to sites of infection or tissue damage.

It is also implicated in various inflammatory conditions, making it a valuable biomarker for studying immune responses and inflammatory diseases.With its reliable performance and broad utility, the Mouse MIP1B ELISA Kit is an essential tool for researchers studying inflammation, immune regulation, and related pathways in mouse models.

Product Name:Mouse Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit
Product Code:MODL00867
Size:96 Assays
Target:MIP1b
Synonyms:CCL4, ACT2, G-26, LAG1, MIP1-B, SCYA4, Chemokine C-C-Motif Ligand 4, Small Inducible Cytokine A4, Homologous To Mouse Mip-1b
Detection Method:Sandwich
Range:12.5-800pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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