null

Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit (MODL00866)

SKU:
MODL00866
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10855
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL3,MIP1-A,SCYA3,Chemokine C-C-Motif Ligand 3,Small Inducible Cytokine A3,Homologous To Mouse Mip-1a,Tonsillar Lymphocyte LD78 Alpha Protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit

The Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1a) ELISA Kit is a reliable and accurate tool for detecting levels of MIP-1a in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MIP-1a is a key player in the immune response, particularly in inflammation and immune cell recruitment. It is involved in various diseases and conditions, including autoimmune disorders, infectious diseases, and inflammatory conditions.

Studying MIP-1a levels can provide valuable insights into these processes and aid in the development of potential therapeutic interventions.Get your Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1a) ELISA Kit today and unlock the potential for groundbreaking research in immunology and inflammation.

Product Name:Mouse Macrophage Inflammatory Protein 1 Alpha (MIP1a) ELISA Kit
Product Code:MODL00866
Size:96 Assays
Target:MIP1a
Synonyms:CCL3, MIP1-A, SCYA3, Chemokine C-C-Motif Ligand 3, Small Inducible Cytokine A3, Homologous To Mouse Mip-1a, Tonsillar Lymphocyte LD78 Alpha Protein
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products