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Mouse Macrophage Derived Chemokine (MDC) ELISA Kit

SKU:
MODL00853
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9R1V4
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL22,ABCD1,DC/B-CK,SCYA22,STCP1,Chemokine C-C-Motif Ligand 22,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22,Stimulated T-Cell Chemotactic Protein 1
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Mouse Macrophage Derived Chemokine (MDC) ELISA Kit

The Mouse Macrophage-Derived Chemokine (MDC) ELISA Kit is a reliable and sensitive tool for the detection of MDC levels in mouse serum, plasma, and cell culture supernatants. With high specificity and accuracy, this kit delivers consistent and precise results, making it suitable for a variety of research purposes.MDC is a chemokine produced by macrophages that plays a crucial role in recruiting immune cells to sites of inflammation. It is known to be involved in various immune responses and inflammatory conditions, making it a valuable biomarker for studying immune system function and inflammation-related diseases.

Overall, the Mouse Macrophage-Derived Chemokine (MDC) ELISA Kit is a vital tool for researchers looking to investigate the role of MDC in immune responses and inflammatory diseases, providing valuable insights for potential therapeutic interventions.

Product Name:Mouse Macrophage Derived Chemokine (MDC) ELISA Kit
Product Code:MODL00853
Size:96 Assays
Target:MDC
Synonyms:CCL22, ABCD1, DC/B-CK, SCYA22, STCP1, Chemokine C-C-Motif Ligand 22, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22, Stimulated T-Cell Chemotactic Protein 1
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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