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Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit

SKU:
MODL00818
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91V01
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MBOAT5,C3F,OACT5,Nessy,LPSAT,Membrane Bound O-AcylTransferase Domain Containing 5,Lysophospholipid Acyltransferase 5,Lysophosphatidylserine acyltransferase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit

The Mouse LPCAT3 (Lysophosphatidylcholine Acyltransferase 3) ELISA Kit is specifically designed for the quantitative measurement of LPCAT3 levels in mouse samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and cell culture supernatants.LPCAT3 is an enzyme that plays a key role in the synthesis of phosphatidylcholine, an essential component of cell membranes.

Dysregulation of LPCAT3 has been linked to various diseases, including metabolic disorders and cancer, making it a valuable biomarker for research and potential therapeutic development.Overall, the Mouse LPCAT3 ELISA Kit is a valuable tool for studying the role of LPCAT3 in physiological and pathological processes, providing researchers with reliable data for their experiments.

Product Name:Mouse Lysophosphatidylcholine Acyltransferase 3 (LPCAT3) ELISA Kit
Product Code:MODL00818
Size:96 Assays
Target:LPCAT3
Synonyms:MBOAT5, C3F, OACT5, Nessy, LPSAT, Membrane Bound O-AcylTransferase Domain Containing 5, Lysophospholipid Acyltransferase 5, Lysophosphatidylserine acyltransferase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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