Mouse Lungkine Recombinant Protein (RPPB1185)
- SKU:
- RPPB1185
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q9WVL7
- Research Area:
- Chemokines
Description
| Product Name: | Mouse Lungkine Recombinant Protein |
| Product Code: | RPPB1185 |
| Size: | 20µg |
| Species: | Mouse |
| Target: | Lungkine |
| Synonyms: | C-X-C motif chemokine 15, Lungkine, Small-inducible cytokine B15, Cxcl15, Scyb15. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Lungkine protein was lyophilized from a 0.2�m filtered concentrated solution in PBS pH 7.4 and 0.02% Tween-20. |
| Solubility: | It is recommended to reconstitute the lyophilized Lungkine in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Lungkine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL15 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | QELRCLCIQE HSEFIPLKLI KNIMVIFETI YCNRKEVIAV PKNGSMICLD PDAPWVKATV GPITNRFLPE DLKQKEFPPA MKLLYSVEHE KPLYLSFGRP ENKRIFPFPI RETSRHFADL AHNSDRNFLR DSSEVSLTGS DA |
| Biological Activity: | The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 20-100 ng/ml. |
Mouse Lungkine/CXCL15 (WECHE) belongs to the ELR motif-containing CXC chemokines. The mouse Lungkine gene has been mapped to chromosome 5. The cDNA of mouse Lungkine encodes a 166 amino acids (aa) protein with a 25 aa predicted signal peptide and a 141 aa mature protein with an exceptionally long C-terminal tail which extends beyond beyond the chemokine fold. Lungkine protein is secreted into bronchoalveolar space and is involved in lung-specific neutrophils trafficking. Furthermore, studies in Lungkine knockout mice propose that Lungkine is an imperative mediator of neutrophil migration from the lung parenchyma into the airspace. In addition, Lungkine is chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation. By Northern blot analysis and in-situ hybridization, Lungkine transcripts have only been specifically detected in the adult and fetal lung, and its expression is up-regulated under inflammatory conditions. There is a 35% aa sequence similarity between the mouse Lungkine and the human ENA-78 and a 31% similarity with the human IL-8.
Recombinant Mouse Lungkine produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 142 amino acids and having a molecular mass of 16.4kDa.The CXCL15 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CXCL15: Chemotactic for neutrophils. May be involved in lung- specific neutrophil trafficking during normal and inflammatory conditions. Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Protein Details: | Protein type:Chemokine Cellular Component: extracellular space; extracellular region Molecular Function:chemokine activity; CXCR chemokine receptor binding; cytokine activity Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; positive regulation of leukocyte chemotaxis; hemopoiesis; response to lipopolysaccharide; immune response; inflammatory response; chemotaxis; regulation of cell proliferation |
| UniProt Code: | Q9WVL7 |
| NCBI GenInfo Identifier: | 7674364 |
| NCBI Gene ID: | 20309 |
| NCBI Accession: | Q9WVL7.1 |
| UniProt Secondary Accession: | Q9WVL7,Q3UQ15, |
| UniProt Related Accession: | Q9WVL7 |
| Molecular Weight: | |
| NCBI Full Name: | C-X-C motif chemokine 15 |
| NCBI Synonym Full Names: | chemokine (C-X-C motif) ligand 15 |
| NCBI Official Symbol: | Cxcl15�� |
| NCBI Official Synonym Symbols: | Il8; weche; Scyb15; lungkine�� |
| NCBI Protein Information: | C-X-C motif chemokine 15; interleukin 8; small-inducible cytokine B15; small inducible cytokine subfamily B, member 15 |
| UniProt Protein Name: | C-X-C motif chemokine 15 |
| UniProt Synonym Protein Names: | Lungkine; Small-inducible cytokine B15 |
| Protein Family: | C-X-C motif chemokine |
| UniProt Gene Name: | Cxcl15�� |
| UniProt Entry Name: | CXL15_MOUSE |
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