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Mouse Low Density Lipoprotein Receptor Related Protein 5 (LRP5) ELISA Kit

SKU:
MODL00825
Product Type:
ELISA Kit
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HBM,BMND1,EVR1,EVR4,LR3,LRP7,OPPG,OPS,OPTA1,VBCH2,Osteoporosis Pseudoglioma Syndrome,Exudative Vitreoretinopathy 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Low Density Lipoprotein Receptor Related Protein 5 (LRP5) ELISA Kit

The Mouse Low Density Lipoprotein Receptor-Related Protein 5 (LRP5) ELISA Kit is specifically designed for the precise measurement of LRP5 levels in mouse serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.LRP5 is a key regulator of the Wnt signaling pathway and is involved in bone metabolism, lipid metabolism, and glucose homeostasis.

Dysregulation of LRP5 has been implicated in several diseases, including osteoporosis, hypercholesterolemia, and type 2 diabetes, making it a valuable target for research and potential therapeutic interventions.Overall, the Mouse LRP5 ELISA Kit offers researchers a reliable tool for studying the role of LRP5 in various physiological and pathological processes, providing valuable insights into potential therapeutic strategies for related disorders.

Product Name:Mouse Low Density Lipoprotein Receptor Related Protein 5 (LRP5) ELISA Kit
Product Code:MODL00825
Size:96 Assays
Target:LRP5
Synonyms:HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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