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Mouse Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

SKU:
MODL00824
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91ZX7
Sensitivity:
0.264ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD91,LRP,A2MR,APOER,APR,TGFBR5,Alpha-2-Macroglobulin Receptor,Prolow-density lipoprotein receptor-related protein 1,Apolipoprotein E receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Mouse Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit

The Mouse Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit is a highly sensitive and specific assay designed for the precise quantification of LRP1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an invaluable tool for researchers investigating the role of LRP1 in various biological processes.LRP1 is a key receptor involved in the clearance of lipoproteins and regulation of cellular signaling pathways. Dysregulation of LRP1 expression has been implicated in a variety of diseases, including Alzheimer's disease, atherosclerosis, and cancer.

By measuring LRP1 levels, researchers can gain insights into the pathophysiological mechanisms underlying these conditions and potentially identify novel therapeutic targets.Overall, the Mouse LRP1 ELISA Kit offers researchers a reliable and efficient method for studying the functions of LRP1 and its implications in disease progression, paving the way for the development of targeted therapies aimed at modulating LRP1 activity.

Product Name:Mouse Low Density Lipoprotein Receptor Related Protein 1 (LRP1) ELISA Kit
Product Code:MODL00824
Size:96 Assays
Target:LRP1
Synonyms:CD91, LRP, A2MR, APOER, APR, TGFBR5, Alpha-2-Macroglobulin Receptor, Prolow-density lipoprotein receptor-related protein 1, Apolipoprotein E receptor
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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