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Mouse Lipase, Hepatic (LIPC) ELISA Kit

SKU:
MODL00805
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P27656
Sensitivity:
0.119ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HL,HTGL,Hepatic Lipase,Hepatic triacylglycerol lipase,Lipase member C
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Lipase, Hepatic (LIPC) ELISA Kit

The Mouse Lipase Hepatic LIPC ELISA Kit is a reliable and efficient tool for the detection of lipase levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in a variety of research applications.Lipase is an important enzyme involved in the breakdown of fats and plays a key role in lipid metabolism. Dysregulation of lipase activity can contribute to various health conditions, including obesity, diabetes, and liver diseases.

Monitoring lipase levels can provide valuable insights into lipid metabolism and potentially aid in the development of therapeutic strategies.Overall, the Mouse Lipase Hepatic LIPC ELISA Kit is a valuable research tool for studying lipid metabolism and related disorders in mouse models, offering researchers a precise and efficient method for analyzing lipase levels.

Product Name:Mouse Lipase, Hepatic (LIPC) ELISA Kit
Product Code:MODL00805
Size:96 Assays
Target:LIPC
Synonyms:HL, HTGL, Hepatic Lipase, Hepatic triacylglycerol lipase, Lipase member C
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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