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Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit

SKU:
MODL00804
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z0M5
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LAL,CESD,Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease),Acid Cholesteryl Ester Hydrolase,Lysosomal acid lipase/cholesteryl ester hydrolase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit

The Mouse Lipase A (Lysosomal Acid Lipa) ELISA Kit is specially designed for the quantitative measurement of Lipase A (Lysosomal Acid Lipa) levels in mouse serum, plasma, tissue homogenates, and other biological fluids. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Lipase A (Lysosomal Acid Lipa) is an essential enzyme involved in lipid metabolism and digestion. It plays a crucial role in breaking down fats into fatty acids and glycerol, contributing to overall metabolic balance.

Dysregulation of Lipase A activity has been associated with various metabolic disorders and diseases, making it a valuable biomarker for studying lipid metabolism and related conditions.Overall, the Mouse Lipase A (Lysosomal Acid Lipa) ELISA Kit provides researchers with a reliable tool for studying the role of Lipase A in health and disease, offering valuable insights into lipid metabolism and potential therapeutic targets.

Product Name:Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit
Product Code:MODL00804
Size:96 Assays
Target:LIPA
Synonyms:LAL, CESD, Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease), Acid Cholesteryl Ester Hydrolase, Lysosomal acid lipase/cholesteryl ester hydrolase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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