The Mouse Lysosomal Acid Lipase A (LIPA) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of LIPA levels in mouse biological samples.
LIPA, known as a critical enzyme involved in cellular lipid metabolism and lysosomal function, plays a pivotal role in lipid digestion and transport within cells. Dysregulation of LIPA activity has been associated with various lipid storage disorders and metabolic abnormalities, underscoring its importance as a biomarker in lipid-related diseases. This ELISA kit empowers researchers to accurately quantify LIPA levels, providing valuable insights into lipid metabolism, lysosomal function, and the pathophysiology of lipid storage disorders. By measuring LIPA concentrations in mouse samples, researchers can gain a deeper understanding of lipid-related disorders, explore potential therapeutic interventions, and assess treatment outcomes targeting LIPA activity. With exceptional sensitivity and specificity, the Mouse LIPA ELISA Kit ensures the generation of reliable and reproducible results, enabling researchers to conduct in-depth studies on LIPA function in mouse models with confidence. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly operation, making it an excellent choice for research investigating lipid metabolism and related disorders in mouse models.
Product Name:
Mouse LIPA (Lipase A, Lysosomal Acid) ELISA Kit
SKU:
AEES00679
Target:
Mouse LIPA (Lipase A, Lysosomal Acid)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LIPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LIPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LIPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LIPA. You can calculate the concentration of Mouse LIPA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
91-102
97-109
Average (%)
101
96
103
1:4
Range (%)
86-100
82-92
86-98
Average (%)
93
86
91
1:8
Range (%)
91-107
80-92
81-95
Average (%)
99
86
88
1:16
Range (%)
90-101
87-101
83-96
Average (%)
95
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
95-108
101
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.54
3.69
0.52
1.48
3.78
Standard deviation
0.03
0.07
0.16
0.03
0.08
0.13
C V (%)
5.56
4.55
4.34
5.77
5.41
3.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LIPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LIPA in samples. No significant cross-reactivity or interference between Mouse LIPA and analogues was observed.
