The Mouse LH (Luteinizing Hormone) ELISA Kit is a sophisticated assay designed for the precise quantitative measurement of LH levels in a variety of biological samples. Luteinizing hormone (LH) plays a vital role in the regulation of reproductive processes, including the stimulation of ovulation in females and the production of testosterone in males. This hormone is crucial for the proper functioning of the ovaries and testes, influencing fertility, sexual development, and overall reproductive health. Accurate measurement of LH levels is essential for understanding the intricate hormonal balance required for normal reproductive function. The Mouse LH ELISA Kit enables researchers to investigate the role of LH in reproductive processes, ovarian and testicular function, and various endocrine disorders.
By quantifying LH levels with precision, this kit provides valuable insights into the hormonal dynamics underlying fertility, puberty, and sexual maturation. With exceptional sensitivity and specificity, the Mouse LH ELISA Kit by Assay Genie ensures reliable and reproducible results, facilitating in-depth research into the physiological and pathological implications of LH dysregulation. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly protocols, making it an ideal choice for scientists seeking to explore the multifaceted functions of Luteinizing Hormone in reproductive biology and endocrinology research. Choose Assay Genie's LH ELISA Kit to advance your investigations and gain a deeper understanding of the complex hormonal factors influencing reproductive health.
Product Name:
Mouse LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00246
Target:
Mouse LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LH. You can calculate the concentration of Mouse LH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-95
89-105
84-99
Average (%)
90
97
92
1:4
Range (%)
92-103
94-101
102-108
Average (%)
95
98
105
1:8
Range (%)
87-93
87-95
82-98
Average (%)
90
92
90
1:16
Range (%)
86-98
101-112
96-104
Average (%)
92
105
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-105
101
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
88-96
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.81
2.24
8.84
0.89
2.37
8.19
Standard deviation
0.04
0.11
0.41
0.05
0.12
0.38
C V (%)
4.94
4.91
4.64
5.62
5.06
4.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LH in samples. No significant cross-reactivity or interference between Mouse LH and analogues was observed.
