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Mouse Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit

SKU:
MODL00798
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8K406
Sensitivity:
0.041ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LGIL4,LGI1-like protein 4,Leucine-rich glioma-inactivated protein 3
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit

The Mouse Leucine-Rich Repeat LGI Family Member 3 (LGI3) ELISA Kit is specifically designed for the accurate measurement of LGI3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and dependable results for a variety of research applications.LGI3 is a key protein involved in cell signaling and has been implicated in neurological disorders such as epilepsy and Alzheimer's disease. By accurately measuring LGI3 levels, researchers can gain valuable insights into the role of this protein in disease pathology and potentially discover new therapeutic targets.

Overall, the Mouse LGI3 ELISA Kit is a valuable tool for researchers studying neurological disorders and seeking to better understand the molecular mechanisms underlying these conditions. Its reliability and accuracy make it an essential component in any research laboratory focused on unraveling the complexities of LGI3 signaling pathways.

Product Name:Mouse Leucine Rich Repeat LGI Family, Member 3 (LGI3) ELISA Kit
Product Code:MODL00798
Size:96 Assays
Target:LGI3
Synonyms:LGIL4, LGI1-like protein 4, Leucine-rich glioma-inactivated protein 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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