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Mouse Left/Right Determination Factor 1 (LEFTY1) ELISA Kit

SKU:
MODL00793
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64280
Sensitivity:
43pg/mL
Range:
125-8000pg/mL
ELISA Type:
Sandwich
Synonyms:
LEFTY-B,LEFTYB,LEFT-B,LEFTB,Left Right Determination Factor B
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Left/Right Determination Factor 1 (LEFTY1) ELISA Kit

The Mouse Left-Right Determination Factor 1 (Lefty1) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of Lefty1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Lefty1 is a key factor in determining left-right asymmetry during embryonic development, playing a critical role in the formation of organs such as the heart and lungs.

Dysregulation of Lefty1 has been linked to a range of developmental abnormalities and diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its reliable performance and ease of use, the Mouse Left-Right Determination Factor 1 (Lefty1) ELISA Kit is an essential tool for researchers studying developmental biology and related fields. Order yours today from Assay Genie to advance your research with confidence.

Product Name:Mouse Left/Right Determination Factor 1 (LEFTY1) ELISA Kit
Product Code:MODL00793
Size:96 Assays
Target:LEFTY1
Synonyms:LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B
Detection Method:Sandwich
Range:125-8,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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