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Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) ELISA Kit

SKU:
MODL00797
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07797
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MAC-2-BP,LGALS3-BP,Galectin-3-Binding Protein,L3 Antigen,Mac-2-Binding Protein,Tumor-associated antigen 90K,Serum Protein 90K,Basement membrane autoantigen p105
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) ELISA Kit

The Mouse LGALS3BP (Lectin, galactoside-binding, soluble 3 binding protein) ELISA Kit is specially designed for the precise measurement of LGALS3BP levels in mouse serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for a variety of research applications.LGALS3BP is a key protein that binds to galectin-3, playing a crucial role in various biological processes such as cell adhesion, migration, and immune response modulation.

Its dysregulation has been linked to conditions like cancer, inflammatory diseases, and cardiovascular disorders, highlighting its significance as a biomarker for diagnostic and therapeutic purposes.In summary, the Mouse LGALS3BP ELISA Kit provides researchers with a powerful tool to investigate the role of LGALS3BP in health and disease, offering valuable insights for potential therapeutic interventions.

Product Name:Mouse Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) ELISA Kit
Product Code:MODL00797
Size:96 Assays
Target:LGALS3BP
Synonyms:MAC-2-BP, LGALS3-BP, Galectin-3-Binding Protein, L3 Antigen, Mac-2-Binding Protein, Tumor-associated antigen 90K, Serum Protein 90K, Basement membrane autoantigen p105
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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