Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit
The Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit is specifically designed for the accurate detection of LDLR levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The low-density lipoprotein receptor (LDLR) is a critical protein involved in the regulation of cholesterol levels in the body, playing a key role in the uptake of LDL cholesterol from the bloodstream.
Dysregulation of LDLR can lead to conditions such as hypercholesterolemia and atherosclerosis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With easy-to-follow instructions and all necessary components included, the Mouse LDLR ELISA Kit from Assay Genie is a valuable tool for researchers investigating the role of LDLR in cardiovascular diseases and lipid metabolism. Order now and take your research to the next level.
Product Name:
Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit
SKU:
MOES01357
Target:
Mouse LDLR (Low Density Lipoprotein Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LDLR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LDLR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LDLR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LDLR. You can calculate the concentration of Mouse LDLR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
88-102
89-102
Average (%)
95
94
95
1:4
Range (%)
91-107
82-94
86-99
Average (%)
99
86
92
1:8
Range (%)
91-109
80-95
86-97
Average (%)
99
87
92
1:16
Range (%)
87-98
85-98
87-99
Average (%)
92
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
90-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.88
4.9
0.54
0.87
4.89
Standard deviation
0.03
0.04
0.27
0.03
0.04
0.16
C V (%)
6
4.55
5.51
5.56
4.6
3.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LDLR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LDLR in samples. No significant cross-reactivity or interference between Mouse LDLR and analogues was observed.
