Mouse Lamin A/C (LMNA) ELISA Kit
- SKU:
- MODL00809
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P48678
- Sensitivity:
- 0.115ng/mL
- Range:
- 0.312-20ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- CDCD1,CMD1A,CMT2B1,EMD2,FPL,FPLD,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,PRO1,Cardiomyopathy Dilated 1A,Limb Girdle Muscular Dystrophy 1B,Progeria 1
- Reactivity:
- Mouse
- Sample Type:
- Tissue Homogenates And Other Biological Fluids
Frequently bought together:
Description
system_update_altDatasheet
Mouse Lamin A/C (LMNA) ELISA Kit
The Mouse Lamin A/C (LMNA) ELISA Kit is specifically designed for the quantitative detection of Lamin A/C levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Lamin A/C is a key structural protein that plays a vital role in maintaining the nuclear envelope integrity and regulating gene expression.
Abnormalities in Lamin A/C have been associated with various genetic disorders, including muscular dystrophies and progeria syndrome. Therefore, this ELISA kit is essential for studying the functions of Lamin A/C and its implications in various diseases, providing valuable insights for potential therapeutic interventions.
| Product Name: | Mouse Lamin A/C (LMNA) ELISA Kit |
| Product Code: | MODL00809 |
| Size: | 96 Assays |
| Target: | LMNA |
| Synonyms: | CDCD1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, Cardiomyopathy Dilated 1A, Limb Girdle Muscular Dystrophy 1B, Progeria 1 |
| Detection Method: | Sandwich |
| Range: | 0.312-20ng/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
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| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
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