The Mouse Klotho ELISA Kit is expertly designed to enable the precise quantitative measurement of Klotho levels in diverse biological samples, focusing on the characterization of this critical protein. Klotho, an essential anti-aging hormone, serves as a modulator of various cellular activities, impacting processes such as cell proliferation, differentiation, and apoptosis. With a pivotal role in regulating aging processes and maintaining homeostasis, Klotho emerges as a vital factor in promoting longevity and attenuating age-related disorders. Accurate determination of Klotho levels is paramount for elucidating its intricate functions in modulating aging-related pathways, including those involved in metabolic disorders, cardiovascular diseases, and renal function. By quantifying Klotho concentrations, researchers can gain crucial insights into its impact on cellular mechanisms, tissue homeostasis, and overall healthspan, providing a deeper understanding of how Klotho influences longevity and age-associated diseases.
The Mouse Klotho ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring dependable and reproducible results for your investigative needs. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it a preferred solution for research studies aiming to uncover the complex roles of Klotho in aging, health, and disease. Opt for Assay Genie's Klotho ELISA Kit to reliably quantify Klotho levels and advance your exploration of the science behind healthy aging and longevity.
Product Name:
Mouse Klotho (Klotho) ELISA Kit
SKU:
AEES00244
Target:
Mouse Klotho (Klotho)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Klotho. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Klotho and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Klotho, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Klotho. You can calculate the concentration of Mouse Klotho in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-104
97-105
91-100
Average (%)
100
101
96
1:4
Range (%)
86-98
96-107
86-99
Average (%)
91
101
93
1:8
Range (%)
95-103
96-104
96-107
Average (%)
99
99
100
1:16
Range (%)
91-101
99-107
88-100
Average (%)
95
104
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-100
97
EDTA plasma (n=5)
87-98
94
Cell culture media (n=5)
89-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.38
103.3
441.2
44.75
107.45
409.89
Standard deviation
2.2
4.75
20.65
2.51
4.91
22.38
C V (%)
5.19
4.6
4.68
5.61
4.57
5.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Klotho concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Klotho in samples. No significant cross-reactivity or interference between Mouse Klotho and analogues was observed.
