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Mouse Kisspeptin Receptor (KISS1R) ELISA Kit

SKU:
MODL00766
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91V45
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AXOR12,GPR54,KiSS1-Derived Peptide Receptor,G Protein-Coupled Receptor 54,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Kisspeptin Receptor (KISS1R) ELISA Kit

The Mouse Kisspeptin Receptor (KISS1R) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of KISS1R levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Kisspeptin receptor, also known as KISS1R, is a key protein involved in regulating the reproductive system and is important for fertility and puberty.

Dysregulation of KISS1R can lead to reproductive disorders and hormonal imbalances, making it a valuable biomarker for studying these conditions and developing potential therapies.By using the Mouse Kisspeptin Receptor (KISS1R) ELISA Kit, researchers can accurately measure KISS1R levels in mouse samples, enabling a better understanding of its role in reproductive health and potential therapeutic interventions.

Product Name:Mouse Kisspeptin Receptor (KISS1R) ELISA Kit
Product Code:MODL00766
Size:96 Assays
Target:KISS1R
Synonyms:AXOR12, GPR54, KiSS1-Derived Peptide Receptor, G Protein-Coupled Receptor 54, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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