The Mouse Kidney Injury Molecule 1 (KIM-1) ELISA Kit is developed for the precise quantification of KIM-1 levels in various biological samples. KIM-1, a cell surface protein expressed in renal tubular cells, serves as a biomarker for kidney injury and inflammation. It plays a vital role in the kidney's response to injury and can indicate damage to the renal system, making it a valuable indicator for renal health evaluation. This ELISA kit offers exceptional sensitivity and specificity, allowing accurate measurement of KIM-1 levels to assess kidney injury and monitor renal function.
With stringent quality control measures, the kit ensures reliable and reproducible results, providing valuable insights into renal pathophysiology. Easy to use and reliable, this kit is an excellent choice for researchers studying kidney diseases and related conditions. (Note: The description includes key information about the kit and its capabilities based on its title and biological context.)
Product Name:
Mouse KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00233
Target:
Mouse KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse KIM-1. You can calculate the concentration of Mouse KIM-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
92-106
84-99
Average (%)
98
100
91
1:4
Range (%)
87-98
88-99
85-99
Average (%)
93
91
92
1:8
Range (%)
89-95
88-97
84-96
Average (%)
92
93
91
1:16
Range (%)
86-101
87-100
85-99
Average (%)
93
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-99
93
EDTA plasma (n=5)
86-98
92
Cell culture media (n=5)
102-109
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.42
1.04
4.86
0.47
1.16
4.38
Standard deviation
0.02
0.05
0.22
0.03
0.06
0.22
C V (%)
4.76
4.81
4.53
6.38
5.17
5.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse KIM-1 in samples. No significant cross-reactivity or interference between Mouse KIM-1 and analogues was observed.
