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Mouse Kallikrein 7 (KLK7) ELISA Kit

SKU:
MODL00776
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91VE3
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PRSS6,SCCE,hK7,Kallikrein-Related Peptidase 7,Serine protease 6,Stratum corneum chymotryptic enzyme
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Kallikrein 7 (KLK7) ELISA Kit

The Mouse Kallikrein 7 (KLK7) ELISA Kit is specifically designed for the precise measurement of KLK7 levels in mouse serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.KLK7 is a key protease enzyme that is associated with various physiological and pathological processes, including skin barrier function, inflammation, and cancer progression.

As such, it serves as an important biomarker for studying skin disorders, immune responses, and cancer development, as well as for potential therapeutic interventions.Overall, the Mouse Kallikrein 7 (KLK7) ELISA Kit is a valuable tool for researchers looking to delve into the mechanisms and implications of KLK7 in mouse models, facilitating a deeper understanding of its role in health and disease.

Product Name:Mouse Kallikrein 7 (KLK7) ELISA Kit
Product Code:MODL00776
Size:96 Assays
Target:KLK7
Synonyms:PRSS6, SCCE, hK7, Kallikrein-Related Peptidase 7, Serine protease 6, Stratum corneum chymotryptic enzyme
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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