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Mouse Kallikrein 6 (KLK6) ELISA Kit

SKU:
MODL00775
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91Y82
Sensitivity:
0.63pg/mL
Range:
1.56-100pg/mL
ELISA Type:
Sandwich
Synonyms:
ZYME,Bssp,Klk7,Neurosin,Zyme,PRSS18,PRSS9,SP59,HK6,Protease M,Serine protease 18
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Cerebrospinal Fluid, Breast Milk And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Kallikrein 6 (KLK6) ELISA Kit

The Mouse Kallikrein 6 (KLK6) ELISA Kit from AssayGenie is a powerful tool for the accurate detection of KLK6 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.KLK6 is a key enzyme in the kallikrein-related peptidase family, playing crucial roles in processes such as nerve regeneration, neuroprotection, and inflammation.

Dysregulation of KLK6 has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.Trust AssayGenie's Mouse KLK6 ELISA Kit to provide reliable and reproducible data, advancing your research efforts in the field of neuroscience and beyond. Order yours today and unlock the potential of KLK6 research.

Product Name:Mouse Kallikrein 6 (KLK6) ELISA Kit
Product Code:MODL00775
Size:96 Assays
Target:KLK6
Synonyms:ZYME, Bssp, Klk7, Neurosin,Zyme, PRSS18, PRSS9, SP59, HK6, Protease M, Serine protease 18
Detection Method:Sandwich
Range:1.56-100pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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