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Mouse Kallikrein 5 (KLK5) ELISA Kit

SKU:
MODL00774
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P15945
Sensitivity:
27pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
KLK-L2,KLKL2,SCTE,Kallikrein-Related Peptidase 5,Kallikrein-like protein 2,Stratum corneum tryptic enzyme
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Kallikrein 5 (KLK5) ELISA Kit

The Mouse KLK5 (Kallikrein 5) ELISA Kit is a powerful tool for detecting KLK5 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.KLK5 is an important enzyme involved in the regulation of skin barrier function and inflammation. Dysregulation of KLK5 has been linked to various skin conditions such as rosacea, eczema, and psoriasis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.

With the Mouse KLK5 ELISA Kit, researchers can confidently measure KLK5 levels in mouse samples, providing valuable insights into skin biology and disease mechanisms. Trust this kit for reliable and reproducible results in your research studies.

Product Name:Mouse Kallikrein 5 (KLK5) ELISA Kit
Product Code:MODL00774
Size:96 Assays
Target:KLK5
Synonyms:KLK-L2, KLKL2, SCTE, Kallikrein-Related Peptidase 5, Kallikrein-like protein 2, Stratum corneum tryptic enzyme
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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