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Mouse Kallikrein 4 (KLK4) ELISA Kit

SKU:
MODL00773
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z0M1
Sensitivity:
0.065ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ARM1,EMSP1,KLK-L1,PRSS17,PSTS,Enamel Matrix,Prostate,Kallikrein-Related Peptidase 4,Enamel matrix serine proteinase 1,Serine protease 17,Kallikrein-like protein 1
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Kallikrein 4 (KLK4) ELISA Kit

The Mouse Kallikrein 4 (KLK4) ELISA Kit is a powerful tool for detecting and quantifying levels of KLK4 in mouse serum, plasma, tissue lysates, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it ideal for various research applications.KLK4 is a member of the kallikrein-related peptidase family, known for its role in various physiological processes, including regulation of inflammation, angiogenesis, and tissue remodeling.

Dysregulation of KLK4 has been implicated in several diseases, such as cancer and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With the Mouse Kallikrein 4 (KLK4) ELISA Kit, researchers can accurately measure KLK4 levels in mouse samples, advancing our understanding of its biological functions and its potential as a therapeutic target.

Product Name:Mouse Kallikrein 4 (KLK4) ELISA Kit
Product Code:MODL00773
Size:96 Assays
Target:KLK4
Synonyms:ARM1, EMSP1, KLK-L1, PRSS17, PSTS, Enamel Matrix,Prostate, Kallikrein-Related Peptidase 4, Enamel matrix serine proteinase 1, Serine protease 17, Kallikrein-like protein 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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