Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit (MOES01558)
- SKU:
- MOES01558
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P05555
- Sensitivity:
- 9.38pg/mL
- Range:
- 15.63-1000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- CD11B, CR3A, MAC-1, MAC1A, MGC117044, MO1A, SLEB6, antigen CD11b(p170), integrin alpha M, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit
- Reactivity:
- Mouse
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
Description
Product Name: | Mouse Itgam/CD11b (Integrin alpha-M) ELISA Kit |
SKU: | MOES01558 |
Target: | Mouse Itgam/CD11b (Integrin alpha-M) |
Size: | 96T |
Assay type: | Sandwich-ELISA |
Assay time: | 3.5h |
Sensitivity: | 9.38 pg/mL |
Detection range: | 15.63-1000 pg/mL |
Kit component: |
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This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Itgam/CD11b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Itgam/CD11b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Itgam/CD11b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Itgam/CD11b. You can calculate the concentration of Mouse Itgam/CD11b in the samples by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse Itgam/CD11b concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes Mouse Itgam/CD11b in samples. No significant cross-reactivity or interference between Mouse Itgam/CD11b and analogues was observed. |