The Mouse Irisin ELISA Kit is designed for the precise quantification of Irisin levels in various biological samples obtained from mouse models. Irisin is a myokine derived from skeletal muscle that plays a significant role in regulating energy metabolism, thermogenesis, and exercise-related benefits in mice. Understanding the levels of Irisin can provide valuable insights into metabolic adaptations, exercise responses, and metabolic disorders in mouse studies. Assay Genie's Mouse Irisin ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results.
Manufactured under stringent quality control guidelines, this kit delivers robust performance and is user-friendly, making it an ideal choice for researchers studying metabolism, energy expenditure, and exercise physiology in mouse models. Trust Assay Genie's Mouse Irisin ELISA Kit for reliable quantification of Irisin levels in your research endeavors.
Product Name:
Mouse Irisin ELISA Kit
SKU:
AEES00197
Target:
Mouse Irisin
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Irisin. You can calculate the concentration of Mouse Irisin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
88-100
99-115
Average (%)
102
95
105
1:4
Range (%)
95-113
92-103
94-108
Average (%)
103
97
101
1:8
Range (%)
96-110
84-99
95-108
Average (%)
102
91
101
1:16
Range (%)
93-105
88-99
99-112
Average (%)
99
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
90-103
96
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
240.52
547.5
2445.42
257.86
500.91
2262.95
Standard deviation
12.17
29.84
130.59
14.85
28.35
113.15
C V (%)
5.06
5.45
5.34
5.76
5.66
5.0
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Irisin concentrations in .
Specificity:
This kit recognizes Mouse Irisin in samples. No significant cross-reactivity or interference between Mouse Irisin and analogues was observed.
