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Mouse Interleukin 8 Receptor Beta (IL-8Rb) ELISA Kit

SKU:
MODL00718
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35343
Sensitivity:
12.4pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD182,CDw128b,IL8R-B,IL8-RB,CMKAR2,CXCR2,IL8R2,C-X-C chemokine receptor type 2,GRO/MGSA receptor,High affinity interleukin-8 receptor B
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Interleukin 8 Receptor Beta (IL-8Rb) ELISA Kit

The Mouse Interleukin-8 Receptor Beta (IL8RB) ELISA Kit is a powerful tool for detecting and quantifying levels of IL8RB in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for researchers in a variety of fields.IL8RB is a key receptor involved in the inflammatory response, playing a critical role in various immune-related processes. Abnormal expression or function of IL8RB has been linked to numerous diseases, including autoimmune disorders and cancer, making it a valuable target for therapeutic intervention and biomarker research.

With its easy-to-use protocol and quick assay time, the Mouse IL8RB ELISA Kit is an essential tool for studying the role of IL8RB in disease pathogenesis and exploring potential therapeutic strategies. Stay ahead in your research with this cutting-edge ELISA kit from Assay Genie.

Product Name:Mouse Interleukin 8 Receptor Beta (IL-8Rb) ELISA Kit
Product Code:MODL00718
Size:96 Assays
Target:IL8Rb
Synonyms:CD182, CDw128b, IL8R-B, IL8-RB, CMKAR2, CXCR2, IL8R2, C-X-C chemokine receptor type 2, GRO/MGSA receptor, High affinity interleukin-8 receptor B
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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