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Mouse Interleukin 3 (IL-3) ELISA Kit

SKU:
MODL00707
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01586
Sensitivity:
7.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
MCGF,MULTI-CSF,MCSF,M-CSF,Multiple,Multilineage-Colony-Stimulating Factor,Hematopoietic Growth Factor,P-Cell Stimulating Factor,Mast-Cell Growth Factor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Interleukin 3 (IL-3) ELISA Kit

The Mouse Interleukin-3 (IL-3) ELISA Kit is specifically designed for the accurate quantification of interleukin-3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Interleukin-3 is a key cytokine known for its role in promoting the growth and differentiation of hematopoietic cells. It plays a vital part in regulating immune responses and inflammation, making it a valuable biomarker for studying various diseases and conditions, including autoimmune disorders, allergies, and hematological malignancies.

With the Mouse Interleukin-3 (IL-3) ELISA Kit, researchers can confidently measure IL-3 levels in mouse samples, advancing their understanding of immune system function and potential therapeutic interventions. Order now to accelerate your research efforts with this reliable and effective tool.

Product Name:Mouse Interleukin 3 (IL-3) ELISA Kit
Product Code:MODL00707
Size:96 Assays
Target:IL3
Synonyms:MCGF, MULTI-CSF, MCSF, M-CSF, Multiple, Multilineage-Colony-Stimulating Factor, Hematopoietic Growth Factor, P-Cell Stimulating Factor, Mast-Cell Growth Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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