null

Mouse Interleukin 2 (IL-2) ELISA Kit

SKU:
MODL00696
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04351
Sensitivity:
6.0pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TCGF,Lymphokine,T-Cell Growth Factor,Aldesleukin
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Mouse Interleukin 2 (IL-2) ELISA Kit

The Mouse Interleukin-2 (IL-2) ELISA Kit is specially designed for accurate detection of interleukin-2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Interleukin-2 is a key cytokine involved in regulating the immune response, specifically in T cell proliferation and differentiation. Dysregulation of IL-2 levels is associated with autoimmune disorders, inflammatory diseases, and cancer.

Therefore, this ELISA kit is valuable for studying the role of IL-2 in these conditions and potentially developing new therapeutic interventions.With this Mouse Interleukin-2 (IL-2) ELISA Kit, researchers can confidently measure IL-2 levels in mouse samples, contributing to a better understanding of immune responses and disease mechanisms.

Product Name:Mouse Interleukin 2 (IL-2) ELISA Kit
Product Code:MODL00696
Size:96 Assays
Target:IL2
Synonyms:TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products